EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.40 | full-length cDNA cloned by PCR | Plexaura homomalla |
EC Number | General Stability | Organism |
---|---|---|
1.13.11.40 | unstable in solution, activity is completely lost after standing on ice overnight | Plexaura homomalla |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.40 | 76000 | - |
mature protein predicted from the cDNA, difference from the size estimated by SDS-PAGE implies a post-translational modification of the enzyme | Plexaura homomalla |
1.13.11.40 | 100000 | - |
SDS-PAGE | Plexaura homomalla |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.40 | 2 arachidonic acid + 2 O2 | Strongylocentrotus purpuratus | - |
8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O | - |
? | |
1.13.11.40 | 2 arachidonic acid + 2 O2 | Asteroidea | - |
8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O | - |
? | |
1.13.11.40 | 2 arachidonic acid + 2 O2 | Callista chione | - |
8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O | - |
? | |
1.13.11.40 | 2 arachidonic acid + 2 O2 | Plexaura homomalla | - |
8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.40 | Asteroidea | - |
starfish | - |
1.13.11.40 | Callista chione | - |
clam, synonym Concha fina | - |
1.13.11.40 | Plexaura homomalla | - |
sea whip coral | - |
1.13.11.40 | Strongylocentrotus purpuratus | - |
sea urchin | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.13.11.40 | additional information | different values obtained from cDNA and SDS-PAGE imply a post-transitional modification | Plexaura homomalla |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.40 | - |
Plexaura homomalla |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.40 | 352 | - |
- |
Plexaura homomalla |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.40 | 2 arachidonic acid + 2 O2 | - |
Strongylocentrotus purpuratus | 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O | - |
? | |
1.13.11.40 | 2 arachidonic acid + 2 O2 | - |
Asteroidea | 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O | - |
? | |
1.13.11.40 | 2 arachidonic acid + 2 O2 | - |
Callista chione | 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O | - |
? | |
1.13.11.40 | 2 arachidonic acid + 2 O2 | - |
Plexaura homomalla | 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O | - |
? | |
1.13.11.40 | arachidonic acid + O2 | - |
Strongylocentrotus purpuratus | (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid | - |
? | |
1.13.11.40 | arachidonic acid + O2 | - |
Asteroidea | (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid | - |
? | |
1.13.11.40 | arachidonic acid + O2 | - |
Callista chione | (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid | - |
? | |
1.13.11.40 | arachidonic acid + O2 | - |
Plexaura homomalla | (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.40 | 19.3 | - |
arachidonic acid | purified enzyme | Plexaura homomalla | |
1.13.11.40 | 66.7 | - |
arachidonic acid | - |
Plexaura homomalla |